Coding

Part:BBa_K2665017:Design

Designed by: You Tong   Group: iGEM18_Kyoto   (2018-10-08)


TDH3-ZrFPS1-6xHis-CYC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1154
    Illegal BamHI site found at 2796
    Illegal XhoI site found at 2651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1134
    Illegal BsaI site found at 1636
    Illegal BsaI site found at 2559
    Illegal BsaI.rc site found at 2654


Design Notes

We added promoter and terminator in order to express the protein, and added His-tag for Western Blotting.


Source

from Z.roxii

References